Protein Isolierung



 Macherey - Nagel 
 
Purification of His-taq Proteins Protino®         
   
   
     
 Bulk Material    
 Product                              Binding Capacity              Format                                                       
 Protino® Ni-TED Resin 10 mg/g Resin
 Protino® Ni-IDA Resin
20 mg/g
Resin
 Protino® Ni-NTA Agarose
50 mg/mL
Resin
   
 
 Single Prep  
 
 Product Binding Capacity 
Format 
 Protino® Ni-TED    150 Packed Columns            0.4 mg
Mini gravity-flow columns
 Protino® Ni-TED 1000 Packed Columns
 2.5 mg
Midi gravity-flow columns 
 Protino® Ni-TED 2000 Packed Columns
 5 mg
Maxi gravity-flow columns
 
 
 
 Protino® Ni-IDA 150 Packed Columns
 0.8 mg
Mini gravity-flow columns
 Protino® Ni-IDA 1000 Packed Columns 
 5 mg
Midi gravity-flow columns 
 Protino® Ni-IDA 2000 Packed Columns 
 10 mg
Maxi gravity-flow columns
 
 
 
 Protino® Ni-NTA Columns 1 mL
 ≤ 50 mg
FPLC™ columns
 Protino® Ni-NTA Columns 5 mL
 ≤ 250 mg
FPLC™ columns
 
 
 
 Manual high throughput (HTP)
 
 
 Product
 
 Format
 Protino® 96 Ni-IDA
 1 mg
96-well gravity-flow plates


 Macherey-Nagel                                               
 
Purification of GST-taq Proteins               
            
   
   
 Single Prep                                                                                
 Product Binding Capacity                   Format                                                       
 Protino GST/4B 
 Columns 1 mL
 10 mg Ready-to-use
1 mL FPLC™ columns
   
 
 Media  
 
 Product   Binding Capacity
 Format
 Protino Glutathione Agarose 4B  > 8 mg/mL
 Resin
   
   
 Applilchem                                              
 
Purification of Proteins 
 
     
 Gel Filtration  
 DextraSEC PRO2  
 DextraSEC PRO10    
 DextraSEC 96W    
 
* Binding capacity will vary for each GST-tagged protein