Klonieren und Protein Expression
We offer a wiede range of chemically competent and electrocompetent cells for cloning, which
include alpha-select Bronze Efficiency Competent Cells for library construction and competent cells
for specialized applications. In general, lower efficiency competent cells are suitable for most purposes,
such as transforming purified plasmid DNA, whereas high-efficiency competent cells are suitable for
more difficult cloning and library construction.Bioline's BL21 competent cells are for protein expression from vectors containing E. coli promotors
and are available for either T7 promotor or non-T7 promotor expression. The E. coli Strain B derived
BL21 competent cells are suitablefor high-level expression of a variety of recombinant proteins, grown
in a minimal media BL21 cells are deficient in key poteases.
In addition to competent cells, this chapter contains additional reagents for cloning such as
ready-to-use antibiotic solutions. Furthermore you find Cuvettes in this chapter.
| Cloning Cells | |
| α-Select Competent Cells | |
| - α-Select Bronze Efficiency | |
| - α-Select Silver Efficiency | |
| - α-Select Gold Efficiency | |
| - α-Select Electrocomponent | |
| α-Select Competent Cells Bacteriophage T1 Resistant | |
| - α-Select Silver Efficiency T1-Resistant | |
| - α-Select Gold Efficiency T1-Resistant | |
| dam-/dcm- Chemically Competent Cells Bacteriophage T1-Resistant | |
| CH3-Blue Chemically Competent Cells | |
| ElectroSHOX Competent Cells | |
| BIOBlue Chemically Competent Cells | |
| Protein Expression | |
| BL21 Competent Cells | |
| - BL21 | |
| - BL21 (DE3) | |
| - BL21(DE3) pLysS | |
| - BL21 (DE3) pLysE | |
| - BL21 Combo Pack | |
| Cuvettes | |
| Cuvettes (Individually wrapped and sterile) | |
| Cloning Reagents | |
| Quick Stick Ligase | |
| T4 DNA Ligase | |
| Uracil DNA Glycosilase | |
| SOC Medium | |
| IPTG & IPTG Solution | |
| X-GAL | |
| Antibiotic Solutions | |
| Antibiotic Solutions | |
| - Ampicilin | |
| - Carbenicilin | |
| - Chloramphenicol | |
| - Kanamycin | |
| - Neomycin | |
| - Tetracyline | |
| Ares Bioscience GeneCraft PCR Enzymes and MIXES |
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| Cloning Reagents | |
| PBS-KSEcoRV-Vector | |
| PBS-SK-T-Vector |
